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当前位置: 首页 > 产品中心 > Ultrapure Polymerases > Enzymatics/Phoenix™ Hot Start Taq DNA Polymerase/P7590L/500 U
公司介绍
Enzymatics is an emerging leader in the supply of the enzymes that drive nucleic acid detection technologies. Our leadership is currently building a team which embodies our mission to lead the molecular biology tools market in quality, customer servi...

Enzymatics/Phoenix™ Hot Start Taq DNA Polymerase/P7590L/500 U

Enzymatics/Phoenix™ Hot Start Taq DNA Polymerase/P7590L/500 U
  • Enzymatics/Phoenix™ Hot Start Taq DNA Polymerase/P7590L/500 U
商品介绍

Phoenix™HotStartTaqDNAPolymerase

ProductDescription

Phoenix™HotStartTaqDNAPolymeraseisarecombinant,ThermostABIleTaqDNApolymerasecomplexedwithathermolabile,neutralizingantibodythatblocksthe5′→3′polymeraseactivitypriortotheinitialDNAdenaturationstepofPCR(1,2).Suchantibody-mediatedHot-Startcapabilityenhancestheoverallspecificity,sensitivityandyieldofthePCRbyreducingnonspecificamplificationandprimer-dimerformationpriortoPCRcycling,andallowstheconvenienceofreactionsetupatroomtemperature.WhenthetemperatureofthePCRreactionmixreaches≥94°CduringtheinitialDNAdenaturingstepofPCRcycling,activityoftheTaqDNApolymeraseisfullyrestored.PhoenixHotStartTaqDNAPolymerase,likestandardTaqDNApolymerase,alsohas5′→3′exonucleaseactivity,butlacksanydetectable3′→5′exonucleaseactivity.

SourceofProtein
Arecombinant E.coli straincarryingtheTaqDNApolymerasegenefromthethermophilicorganismThermusAquaticusYT-1complexedwithamonoclonalantibodyderivedfrommurinecellculture.

Suppliedin
20mMTris-HCl
100mMNaCl
0.1mMEDTA
Stabilizer
50%Glycerol
pH7.5@25°C

SuppliedWith:
5XPhoenixHotStartTaqReactionBuffer(B7590)
5XPhoenixHotStartTaqGCReactionBuffer(B7591)

UnitDefinition
1unitisdefinedastheamountofenzymethatwillincorporate10nmolofdNTPintoacid-insolublematerialin30minutesat75°C.

QualityControlAnalysis

FunctionalAssay
PhoenixHotStartTaqDNApolymerasewastestedforitsabilitytoamplifyDNAtargetsbyPCRinreactionbuffersB7590andB7591followinga24hourincubationatroomtemperature.AfterheatactivationandPCRamplificationthesamplesresultedinvisIBLesinglebandampliconsasdeterminedbyagarosegelelectrophoresis.

TaqInhibition
ThisassaymeasuredtheresidualactivityofPhoenixHotStartTaqDNApolymeraseintheabsenceofa≥94°Cheatactivationstep.PhoenixHotStartTaqDNApolymeraseandTaqDNAPolymerase(TaqDNAPolymerasewithoutTaqAntibody)wereincubatedinthepresenceofCalfThymusDNA,50µM 3H-dTTP,100µMdNTPsand1XreactionbufferB7590orB7591.Aftera24hourincubationatroomtemperaturethesampleswereanalyzedfortotal3H-dTTPcountsincorporatedusingthemethodofSambrookandRussell(MolecularCloning,v3,2001,pp.A8.25-A8.26).

ContaminationTests

Single-StrandedExonucleaseActivity
RADIolabeledsingle-strandedDNAsubstrateincubatedwithenzymesolutionfor4hoursat37°Cresultedinlessthan5%releaseofTCA-solublecounts.

Double-StrandedExonucleaseActivity
Radiolabeleddouble-strandedDNAsubstrateincubatedwithenzymesolutionfor4hoursat37°Cresultedinlessthan1%releaseofTCA-solublecounts.

Double-StrandedEndonucleaseActivity
AreactioncontainingplasmidDNAincubatedwithenzymesolutionfor4hoursat37°CresultedinnovisuallydiscernibleconversiontonickedcircularDNAasdeterminedbyagarosegelelectrophoresis.

ProductInformation

Phoenix™HotStartTaqDNAPolymerase
PartNumberP7590L
Price$213
Concentration5,000U/ml
UnitSize500U
SDSAvailableonrequest

ProductSpecification*

StorageTemperature-25to-15°C
TestSpecification
SpecificActivity,TaqDNAPolymerase(P7580)74,625U/mg
SSExonuclease50U<10%
DSExonuclease50U<1%
DSEndonuclease50U=Noconversion
TaqInhibitionPass
FunctionalAssayFunctionalwithbuffersB7590andB7591

*Foradetailedsummaryofassayconditionsanddata,refertotheQualityControlsAnalysissection.

AdditionalDownloads

References

  1. Chou,Q.etal.(1992)NucleicAcidsResearch20(7),1717-1723.
  2. Sharkey,D.etal.(1994),NatureBiotechnology,12,506–509.

LimitationsofUse
Thisproductwasdeveloped,manufactured,andsoldforinvitrouseonly.TheproductisnotsuitableforadmiNISTrationtohumansoranimals.SDSsheetsrelevanttothisproductareavailableuponrequest.

品牌介绍

Enzymatics is an emerging leader in the supply of the enzymes that drive nucleic acid detection technologies. Our leadership is currently building a team which embodies our mission to lead the molecular biology tools market in quality, customer service, and innovation. We seek engaging, motivated individuals who can articulate their goals and, in turn, offer a culture that breeds success. Enzymatics is an employee-focused organization that strives to cultivate challenging careers and rewarding lives. If you seek a challenge, have a thirst for knowledge, and are interested in having fun while working, please apply to learn more!

We offer a competitive benefits package and an incentive-based compensation package.



苏州蚂蚁淘生物科技有限公司是一家创新型高科技生物公司,以常规生物学实验外包与生物学试剂为基础,放眼于生命科学领域的前沿技术服务,公司技术骨干有着多年的生物学科研背景,毕业于国内外名牌大学,并且有着丰富的项目经验,了解国内外科研行情,我公司力求为每一个客户提专业、针对性的实验方案。我公司以技术服务为支点,和国内外多家生物试剂公司有着密切的业务往来.公司特色技术服务包括:抗体药物研发、噬菌体抗体库、蛋白表达与传化、抗体制备、高通量测序、组学研究、生物信息学分析与方法建立、基础分子生物学技术外包、化学中间体合成、原料药物研发、论文编写等。我公司秉承”诚信做人,认真做事,服务至上“,希望用我们的专业知识和项目经验为国内科研客户解决科研问题,祝您科研之路畅通无阻!


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