请使用支持JavaScript的浏览器!
主营:连接蛋白、DNA修饰酶、NGS文库制备(NGS Library Construction)、核酸和RNA酶。
banner
当前位置: 首页 > 产品中心 > Ultrapure Polymerases > Enzymatics/T4 DNA Polymerase/P7080L/2,000 U
公司介绍
Enzymatics is an emerging leader in the supply of the enzymes that drive nucleic acid detection technologies. Our leadership is currently building a team which embodies our mission to lead the molecular biology tools market in quality, customer servi...

Enzymatics/T4 DNA Polymerase/P7080L/2,000 U

Enzymatics/T4 DNA Polymerase/P7080L/2,000 U
  • Enzymatics/T4 DNA Polymerase/P7080L/2,000 U
商品介绍

T4DNAPolymerase

ProductDescription

T4DNAPolymerasecatalyzestheextensionofaprimedDNAtemplateinthe5′→3′direction.Thisenzymeexhibitsapowerful3′→5′exonucleaseactivity,whilelackinganyinherent5′→3′exonucleaseorstranddisplacementfunctions.

SourceofProtein
Purifiedfromastrainof E.coli thatexpressestherecombinantT4DNAPolymerasegene.

Suppliedin
100mMKPO4
1.0mMDTT
0.1mMEDTA
50%glycerol
pH6.5@25°C

SuppliedWith
B0110(10XBlueBuffer)

10XBlueBuffer(B0110)
500mMNaCl
100mMTris-HCl
100mMMgCl2
10mMDTT
pH7.9@25°C

UnitDefinition
Oneunitisdefinedastheamountofenzymethatwillincorporate10nmolofdNTPintoacid-precipitablematerialin30minutesat37°C.

QualityControlAnalysis

UnitCharacterizationAssay
Unitactivitywasmeasuredusinga2-foldserialdilutionmethod.Dilutionsofenzymeweremadein1Xreactionbufferandaddedto50µLreactionscontainingCalfThymusDNA,1XBlueBuffer, 3H-dTTPand100µMdNTPs.Reactionswereincubated10minutesat37°C,plungedonice,andanalyzedusingthemethodofSambrookandRussell(MolecularCloning,v3,2001,pp.A8.25-A8.26)

ProteinConcentration(OD280)Measurement
A2.0µLsampleofenzymewasanalyzedatOD280 usingaNanodropND-1000spectrophotometerstandardizedusinga2.0mg/mlBSAsample(PierceCat#23209)andblankedwithproductstoragesolution.Theobservedaveragemeasurementof3replicatesampleswasconvertedtomg/mLusinganextinctioncoefficientof128,440andmolecularweightof103,609Daltons.

SDS-Page(PhysicalPurityAssessment)
2.0µLofenzymesolutionwasloadedonadenaturing4-20%Tris-GlycineSDS-PAGEgelflankedbyabroad-rangeMWMarkerand2.0µLofa1:100dilutionofthesample.Followingelectrophoresis,thegelwasstainedandthesamplescomparedtodeterminephysicalpurity.Theacceptancecriteriaforthistestrequiresthattheaggregatemassofcontaminantbandsintheconcentratedsampledonotexceedthemassoftheproteinofinterestbandinthedilutesample,confirminggreaterthan99%purityoftheconcentratedsample.

ContaminationTests

Single-StrandedExonucleaseActivity
A50µLreactioncontaining10,000cpmofarADIolabeledsingle-strandedDNAsubstrateand10µLofenzymesolutionincubatedfor4hoursat37°Cresultedingreaterthan80%releaseofTCA-solublecounts.

Double-StrandedExonucleaseActivity
A50µLreactioncontaining5,000cpmofaradiolabeleddouble-strandedDNAsubstrateand10µLofenzymesolutionincubatedfor4hoursat37°Cresultedingreaterthan50%releaseofTCA-solublecounts.

Double-StrandedEndonucleaseActivity
A50µLreactioncontaining0.5µgofpBR322DNAand10µLofenzymesolutionincubatedfor4hoursat37°CresultedinnovisuallydiscernIBLeconversiontonickedcircularDNAasdeterminedbyagarosegelelectrophoresis.

E.coli16SrDNAContaminationTest
Replicate5µLsamplesofenzymesolutionweredenaturedandscreenedinaTaqManqPCRassayforthepresenceofcontaminatingE.coligenomicDNAusingoligonucleotideprimerscorrespondingtothe16SrRNAlocus.Theacceptancecriterionforthetestisthethresholdcyclecount(Ct)producedbytheaverageof3replicatenotemplatecontrolsamples.BasedonthecorrelationbetweenthenotemplatecontrolCtvalues,andstandardcurvedata,thedetectionlimitofthisassayis<10copiesgenome/sample.

ProductInformation

T4DNAPolymerase
PartNumberP7080L
Price$363
Concentration3,000U/ml
UnitSize2,000U
SDSAvailableonrequest

ProductSpecification*

StorageTemperature-15to-25°C
TestSpecification
Purity(SDS-PAGE)>99%
SpecificActivity5,555U/mg
SSExonucleaseFunctional
DSExonucleaseFunctional
DSEndonuclease30U=noconversion
E.coliDNAContamination30U<10copies

*Foradetailedsummaryofassayconditionsanddata,refertotheQualityControlsAnalysissection.

References

  1. Tabor,S.andStruhl,K.(1989)InDNA-DependentDNAPolymerases.F.M.Ausebel,R.Brent,R.E.Kingston,D.D.Moore,J.G.Seidman,J.A.SmithandK.Struhl(Eds.),CurrentProtocolsinMolecularBIOLOGy,pp.3.5.10-3.5.12.2.Sambrook,J.,Fritsch,E.F.andManiatis,T.(1989)MolecularCloning:ALaboratoryManual,(2ndEd.),5.44-5.47.
  2. Dale,R.,McClure,B.andHouchins,J.(1985)Plasmid,13,31-40.
  3. Kunkel,T.A.,Roberts,J.D.andZakour,R.A.(1987)R.WuandL.Grossman(Eds.),MethodsEnzymol.,154,pp.367-382.SanDiego:AcademicPress.
  4. Panet,A.,vandeSande,J.H.,Loewen,P.C.andKhorana,H.G.(1973)Biochemistry,12,5045-5050.

LimitationsofUse
Thisproductwasdeveloped,manufactured,andsoldforinvitrouseonly.TheproductisnotsuitableforadmiNISTrationtohumansoranimals.SDSsheetsrelevanttothisproductareavailableuponrequest.

品牌介绍

Enzymatics is an emerging leader in the supply of the enzymes that drive nucleic acid detection technologies. Our leadership is currently building a team which embodies our mission to lead the molecular biology tools market in quality, customer service, and innovation. We seek engaging, motivated individuals who can articulate their goals and, in turn, offer a culture that breeds success. Enzymatics is an employee-focused organization that strives to cultivate challenging careers and rewarding lives. If you seek a challenge, have a thirst for knowledge, and are interested in having fun while working, please apply to learn more!

We offer a competitive benefits package and an incentive-based compensation package.



苏州蚂蚁淘生物科技有限公司是一家创新型高科技生物公司,以常规生物学实验外包与生物学试剂为基础,放眼于生命科学领域的前沿技术服务,公司技术骨干有着多年的生物学科研背景,毕业于国内外名牌大学,并且有着丰富的项目经验,了解国内外科研行情,我公司力求为每一个客户提专业、针对性的实验方案。我公司以技术服务为支点,和国内外多家生物试剂公司有着密切的业务往来.公司特色技术服务包括:抗体药物研发、噬菌体抗体库、蛋白表达与传化、抗体制备、高通量测序、组学研究、生物信息学分析与方法建立、基础分子生物学技术外包、化学中间体合成、原料药物研发、论文编写等。我公司秉承”诚信做人,认真做事,服务至上“,希望用我们的专业知识和项目经验为国内科研客户解决科研问题,祝您科研之路畅通无阻!


Enzymatics专注于于酶产品的研发,为市场带来了特殊的、高品质的产品。是一家试剂、试剂盒和测定的领先生产商,使全球客户变革了诊断、治疗和生命科学的研究。业务包括:试剂、供应链解决方案,应用科学,其测序产品市场占有率达80%。


酶科技公司产品包括八类:超纯连接酶如T4连接酶、超纯poly酶、ArcherTM系列测序工具(Archer Products)、连接蛋白、DNA修饰酶、NGS文库制备(NGS Library Construction)、核酸和RNA酶。


Enzymatics is pleased to announce the launch of ZipScript™ One-Step RT-qPCR mix, a high efficiency RNA-dependent multiplex qPCR formulation.


ZipScript, featuring Enzymatics’ own blend of RNAse H minus reverse transcriptase (EnzScript) and fast-activating hot start DNA polymerase (Phoenix Hot Start Taq), delivers users an extremely sensitive RT-qPCR mix in a single tube format.


产品列表:


No.

品牌

货号

名称

规格

1

Enzymatics

N2070-10-L

10 mM ATP Solution 

50 µmol

2

Enzymatics

N2050-10-L

10 mM dNTP Solution Mix

100 µmol

3

Enzymatics

N2010L

100 mM 75µmol dNTP Solution Set 

75 µmol

4

Enzymatics

N2010-A-L

100 mM dATP Solution 

75 µmol

5

Enzymatics

N2050L

100µmol dNTP Solution Mix 

100 µmol

6

Enzymatics

Y9180L

10x Uracil Cleavage System 

750 Reactions

7

Enzymatics

P7670

2x HiFi PCR Master Mix 

1 rxn

8

Enzymatics

Y9420L

5x ER/A-Tailing Enzyme Mix 

24 reactions

9

Enzymatics

Y9410L

5X WGS Fragmentation Mix 

24 Reactions

10

Enzymatics

P7390L

Bst X DNA Polymerase 

40,000 U

11

Enzymatics

P7050L

DNA Polymerase I 

5,000 U

12

Enzymatics

L6090L

E. coli DNA Ligase 

2,500 U

13

Enzymatics

Y9070L

E. coli fpg 

4,000 U

14

Enzymatics

Y9380L

E. coli Pyrophosphatase 

50 U

15

Enzymatics

Y9030L

E. coli Single-Stranded DNA Binding Protein 

1.5 mg


自营商城图标
厂家直采
全球直采模式 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔
在线客服
客服电话

4000-520-616

0512-67156496