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主营:连接蛋白、DNA修饰酶、NGS文库制备(NGS Library Construction)、核酸和RNA酶。
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公司介绍
Enzymatics is an emerging leader in the supply of the enzymes that drive nucleic acid detection technologies. Our leadership is currently building a team which embodies our mission to lead the molecular biology tools market in quality, customer servi...

Enzymatics/Taq-B DNA Polymerase/P7250L/10,000 U

Enzymatics/Taq-B DNA Polymerase/P7250L/10,000 U
  • Enzymatics/Taq-B DNA Polymerase/P7250L/10,000 U
商品介绍

Taq-BDNAPolymerase

ProductDescription

Taq-BDNAPolymeraseisathermallystable,processive,5′→3′DNApolymerase.The94kDaproteinpossessesaninherent5′→3′nick-translationmoietyandlacksa3′→5′proofreADIngfunction.

SourceofProtein
Arecombinant E.coli straincarryingtheTaqDNApolymerasegenefromtheThermophilicorganism ThermusAquaticus YT-1.

Suppliedin
20mMTris-HCl
100mMNaCl
1.0mMDTT
0.1mMEDTA
StABIlizer
50%glycerol
pH7.5@25°C

SuppliedWith
B7030(10XPCRBufferI)

10XPCRBufferl(B7030)
100mMTris-HCl 
500mMKCl
15mMMgCl2
pH8.3@25°C

UnitDefinition
1unitisdefinedastheamountofenzymethatwillincorporate10nmolofdNTPintoacid-insolublematerialin30minutesat75°C.

QualityControlAnalysis

UnitCharacterizationAssay
Specificactivitywasmeasuredusinga2-foldserialdilutionmethod.Dilutionsofenzymeweremadein1Xreactionbufferandaddedto50µLreactionscontainingCalfThymusDNA,25mMTAPS(pH9.3),50mMKCl,2.0mMMgCl2,1mMDTT, 3H-dTTPand100µMdNTPs.Reactionswereincubated10minutesat75°C,plungedonice,andanalyzedusingthemethodofSambrookandRussell(MolecularCloning,v3,2001,pp.A8.25-A8.26)

ProteinConcentration(OD280)Measurement
A2.0µLsampleofenzymewasanalyzedatOD280 usingaNanodropND-1000spectrophotometerstandardizedusinga2.0mg/mlBSAsample(PierceCat#23209)andblankedwithproductstoragesolution.Theobservedaveragemeasurementof3replicatesampleswasconvertedtomg/mLusinganextinctioncoefficientof110,380andmolecularweightof93,910Daltons

SDS-Page(PhysicalPurityAssessment)
2.0µLofconcentratedenzymesolutionwasloadedonadenaturing4-20%Tris-GlycineSDS-PAGEgelflankedbyabroad-rangeMWMarkerand2.0µLofa1:100dilutionofthesample.Followingelectrophoresis,thegelwasstainedandthesamplescomparedtodeterminephysicalpurity.Theacceptancecriteriaforthistestrequiresthattheaggregatemassofcontaminantbandsintheconcentratedsampledonotexceedthemassoftheproteinofinterestbandinthedilutesample,confirminggreaterthan99%purityoftheconcentratedsample.

ContaminationTests

Single-StrandedExonucleaseActivity
A50µlreactioncontaining10,000cpmofaradiolabeledsingle-strandedDNAsubstrateand10µLofenzymesolutionincubatedfor4hoursat37°Cresultedinlessthan5.0%releaseofTCA-solublecounts.

Double-StrandedExonucleaseActivity
A50µlreactioncontaining5,000cpmofaradiolabeleddouble-strandedDNAsubstrateand10µLofenzymesolutionincubatedfor4hoursat37°Cresultedinlessthan1.0%releaseofTCA-solublecounts.

Double-StrandedEndonucleaseActivity
A50µLreactioncontaining0.5µgofpBR322DNAand10µLofenzymesolutionincubatedfor4hoursat37°CresultedinnovisuallydiscernIBLeconversiontonickedcircularDNAasdeterminedbyagarosegelelectrophoresis.

E.coli 16SrDNAContaminationTest
Replicate5uLsamplesofenzymesolutionweredenaturedandscreenedinaTaqManqPCRassayforthepresenceofcontaminating E.coli genomicDNAusingoligonucleotideprimerscorrespondingtothe16SrRNAlocus.Theacceptancecriterionforthetestisthethresholdcyclecount(Ct)producedbytheaverageof3replicatenotemplatecontrolsamples.BasedonthecorrelationbetweenthenotemplatecontrolCt values,andstandardcurvedata,thedetectionlimitofthisassayis<10copiesgenome/sample.

 

ProductInformation

Taq-BDNAPolymerase
PartNumberP7250L
Price$363
Concentration5,000U/ml
UnitSize10,000U
SDSAvailableonrequest

ProductSpecification*

StorageTemperature-25to-15°C
TestSpecification
Purity(SDS-PAGE)>99%
SpecificActivity74,625U/mg
SSExonuclease50U<5.0%released
DSExonuclease50U<1.0%released
DSEndonuclease50U=Noconversion
E.coliDNAContamination50U<10copies

*Foradetailedsummaryofassayconditionsanddata,refertotheQualityControlsAnalysissection.

UsageInstructions

Typical50µLReaction
Onice,prepareeachoffollowingmastermixes,combine,andplaceinheated(to94°C)thermalcycler:

2XDNA/OligonucleotideMasterMix:
1.0µL10mMdNTPs
1.0µL10µMForwardPrimer
1.0µL10µMReversePrimer
1.0µL500ng/µLgenomicDNA
21µLTypeIWater

2XEnzyme/BufferMasterMix:
5.0µL10XPCRBufferI
0.2µL5U/µLTaqDNAPolymerase
19.8µLTypeIWater

GeneralCyclingConditions
94°C      3minutesInitialDenaturation
25Cycles:
94°C      30seconds            Denaturation
55°C      30seconds            Annealing
68°C      30seconds           500bpextension
68°C      5minutes               FinalExtension

Notes

TaqDNAPolymeraseistheoriginalandmostcommonlyusedPCRenzyme.Taqexcelsatamplifyingshorter(<5kb)sequencesfromlow-complexitytemplatesourcesandproducesrobustyieldswithlittleornooptimizationofreactionconditions.ConsiderthefollowingguidelineswhendesigningPCRstrategiesusingTaqDNAPolymerase.

  1. DNATemplate: AlthoughextensivepurificationofPCRtemplatesistypicallynotnecessary,careshouldbetakenwithcrudeorpartiallypurifiedDNAsourcesashandlingandchemicalagentscanadverselyaffectthePCRprocess.Exposuretoshort-waveUVlightorotherDNAdamagingagentsshouldbeavoided,asshouldhighionicstrength,detergentssuchasSDS,loadingdyesandphenol.InordertopreventcontaminationfrompreviousPCRreactions,considersettingupreactionsinapositive-pressurehoodandwithaerosolbarrierpipettips.Inatypical25cyclePCR,104copiesoftargetsequencewillyieldreproducibleamplificationproduct.Thiscorrespondstoroughly0.1-1ng/ml(finalconcentration)ofplasmidDNA,and1-10µg/mlofgenomicDNA.TheuseoflowerDNAconcentrationstypicallyproduceslessnon-specificproduct,whilehigherconcentrationscanallowforfewercyclesandlowermutationrates.
  2. PrimerDesign:IIdeally,oligonucleotideprimersare15-30basesinlength,nearly50%G+C,andhaveequal(+/-3°C)annealingtemperatures.Theuseofsoftwaretodetectself-complementaryorhairpin-proneregionsisadvisedandisofferedasaservicebysomesynthesisproviders.Notethatalthoughthe5′-terminusoftheprimermaycontainuntemplatedsequence,the3&primeendmustmatchperfectly.Typicaloligonucleotideconcentrationinthereactionis0.1-0.5µM.
  3. Magnesium:MagnesiumisacriticalcomponentofthePCRreactionthoughitsconcentrationcanbemodulatedtopromotevariouseffects.Generally,1.5-2.0mMMg2+istargeted,buthigherconcentrations(upto5mM)maybeusedtostimulatetheyieldofreactionsattheexpenseoffidelity.Theconverseisalsotrue–lowermagnesiumconcentrationswillpromotehigher-fidelityproductswithaloweroverallamplificationyield.Notethatcertainreactioncomponents,inparticulartemplateDNAandoligonucleotides,maycontributechelatingagentstothereactionwhichcouldlowertheeffectivemagnesiumconcentrationandstarvethereaction.
  4. dNTPs:Generally,afinalconcentrationof100-200µMdNTPsisemployed,thoughhigherconcentrationsmaystimulateyields(particularlywithlongertargets)andlowermayofferincreasesinfidelity.TaqDNAPolymerasecanalsoincorporateandreadthroughdeoxyUridineandInosine,twoanalogsusedincertainapplications.
  5. TaqPolymerase:1unit/50µLreaction(20U/mL)istypical,thoughadditionalenzymemaybeaddedtostimulateyields.TaqDNAPolymeraseextendsaDNAtemplateatapproximately2000nucleotides/minute,soitisrecommendedthat45-60secondsofextensiontimebeprovidedpercycle.Appropriateextensiontemperaturesrangefrom66-72°C.

LegalDisclaimer

Patents
CertainapplicationsinwhichthisproductcanbeusedmaybecoveredbypatentsissuedandapplicableintheUnitedStatesandabroad.Purchaseofthisproductdoesnotincludealicensetoperformanypatentedapplication,thereforeitisthesoleresponsibilityofusersofthisproducttodeterminewhethertheymayberequiredtoengagealicenseagreementdependingupontheparticularapplicationinwhichtheproductisused.

LimitationsofUse
Thisproductwasdeveloped,manufactured,andsoldforinvitrouseonly.TheproductisnotsuitableforadmiNISTrationtohumansoranimals.SDSsheetsrelevanttothisproductareavailableuponrequest.

品牌介绍

Enzymatics is an emerging leader in the supply of the enzymes that drive nucleic acid detection technologies. Our leadership is currently building a team which embodies our mission to lead the molecular biology tools market in quality, customer service, and innovation. We seek engaging, motivated individuals who can articulate their goals and, in turn, offer a culture that breeds success. Enzymatics is an employee-focused organization that strives to cultivate challenging careers and rewarding lives. If you seek a challenge, have a thirst for knowledge, and are interested in having fun while working, please apply to learn more!

We offer a competitive benefits package and an incentive-based compensation package.



苏州蚂蚁淘生物科技有限公司是一家创新型高科技生物公司,以常规生物学实验外包与生物学试剂为基础,放眼于生命科学领域的前沿技术服务,公司技术骨干有着多年的生物学科研背景,毕业于国内外名牌大学,并且有着丰富的项目经验,了解国内外科研行情,我公司力求为每一个客户提专业、针对性的实验方案。我公司以技术服务为支点,和国内外多家生物试剂公司有着密切的业务往来.公司特色技术服务包括:抗体药物研发、噬菌体抗体库、蛋白表达与传化、抗体制备、高通量测序、组学研究、生物信息学分析与方法建立、基础分子生物学技术外包、化学中间体合成、原料药物研发、论文编写等。我公司秉承”诚信做人,认真做事,服务至上“,希望用我们的专业知识和项目经验为国内科研客户解决科研问题,祝您科研之路畅通无阻!


Enzymatics酶产品

中文名称 Product Name Part No. Unit Size
EnzScript? (MMLV 逆转录酶,RNase H-)  EnzScript?  (MMLV Reverse Transcriptase RNase H-)  P7600L 10,000  U
Poly(A) 聚合酶 Poly(A)  Polymerase  P7460L 1,000  U
核酸内切酶 RNAse  H Y9220L  5,000  U
Y9220F  770  U
RNA酶抑制剂 RNAse  Inhibitor Y9240L 20,000  U
Archer? Barcode 适配体1-8,用于Ion Torrent?平台, Archer?  Barcode Adapters 1-8 For Ion Torrent? Platform  SA0027 N/A
Archer? Barcode 适配体17-24,用于Ion Torrent?平台, Archer?  Barcode Adapters 17-24 For Ion Torrent? Platform  SA0029 N/A
Archer? Barcode 适配体33-40,用于Ion Torrent?平台, Archer?  Barcode Adapters 33-40 For Ion Torrent? Platform  SA0031 N/A
Archer? Barcode 适配体41-48,用于Ion Torrent?平台, Archer?  Barcode Adapters 41-48 For Ion Torrent? Platform  SA0032 N/A
Archer? Barcode 适配体9-16,用于Ion Torrent?平台, Archer?  Barcode Adapters 9-16 For Ion Torrent? Platform  SA0028 N/A
Archer? FusionPlex? ARR第二代面板 Archer?  FusionPlex? ARR Panel v2  AK0028-8 8  reactions
Archer? FusionPlex? FGFR面板 Archer?  FusionPlex? FGFR Panel  AK0030-8 8  Reactions
Archer? FusionPlex? 血红素面板 Archer?  FusionPlex? Heme Panel  AK0029-8 8  reactions
Archer? FusionPlex? NTRK 面板 Archer?  FusionPlex? NTRK Panel  AK0031-8 8  reactions
Archer? FusionPlex? 肉瘤面板 Archer?  FusionPlex? Sarcoma Panel  AK0032-8 8  reactions
Archer? MBC 适配体系B,用于Illumina?  Archer?  MBC Adapter Set B for Illumina?  AK0016-48 N/A
Archer? MBC 适配体系C,用于Illumina?  Archer?  MBC Adapter Set C for Illumina?  AK0017-48 N/A
Archer? MBC 适配体系A1-A8,用于Illumina?  Archer?  MBC Adapters A1-A8 for Illumina?  SA0040 N/A
Archer? MBC 适配体系A17-A24,用于Illumina?  Archer?  MBC Adapters A17-A24 for Illumina?  SA0042 N/A
Archer? MBC 适配体系A25-A32,用于Illumina?  Archer?  MBC Adapters A25-A32 for Illumina?  SA0043 N/A
Archer? MBC 适配体系A33-A40,用于Illumina?  Archer?  MBC Adapters A33-A40 for Illumina?  SA0044 N/A
Archer? MBC 适配体系A41-A48,用于Illumina?  Archer?  MBC Adapters A41-A48 for Illumina?  SA0045 N/A
Archer? MBC 适配体系 A9-A16,用于Illumina?  Archer?  MBC Adapters A9-A16 for Illumina?  SA0041 N/A
Archer? 通用RNA 试剂盒,适用于 Illumina?   Archer?  Universal RNA Reagent Kit for Illumina?  AK0024-8 N/A
Archer? 通用RNA 试剂盒,适用于Ion Torrent?  Archer?  Universal RNA Reagent Kit for Ion Torrent?  AK0025-8 N/A
大肠杆菌单链DNA连接蛋白 E.  coli Single-Stranded DNA Binding Protein  Y9030L 1.0  mg
T4噬菌体基因32编码蛋白 T4  Gene 32 Protein  Y9130L 1.0  mg
10x 尿嘧啶切割系统 10x  Uracil Cleavage System  Y9180L 750  Reactions
大肠杆菌fpg E.  coli fpg  Y9070L 4,000  U
末端修复混合液(高浓度) End-Repair  Mix (High Concentration)  Y9140-HC-L 75  Reactions
末端修复混合液(低浓度) End-Repair  Mix (Low Concentration)  Y9140-LC-L 200  Reactions
 核酸内切酶VIII  Endonuclease  VIII  Y9080L 10,000  U
核酸外切酶I Exonuclease  I X8010L  30,000  U
X8010F  4,600  U
 核酸外切酶III Exonuclease  III X8020L  50,000  U
X8020F  7,700  U
λ-核酸外切酶 Lambda Exonuclease X8030L 10,000  U
RecA  RecA  Y9260L 1.5  mg
T4 DNA多聚核苷酸激酶  T4  Polynucleotide Kinase  Y9040L 10,000  U
热分解尿嘧啶糖苷酶 Thermolabile  UDG  G5020L 500  U
热稳定焦磷酸酶 Thermostable  Pyrophosphatase Y9370L  1,250  U
Y9370F  250  U
尿嘧啶DNA糖苷酶 Uracil  DNA Glycosylase G5010L  10,000  U
G5010F  1,540  U
NGS库预备: 2X  VeraSeq? PCR Mix – 250 Reactions NGS  Library Prep: 2X VeraSeq? PCR Mix – 250 Reactions P7610L  250  Reactions
P7610S  50  Reactions
SPARK? DNA 样品预备盒,用于Illumina?  SPARK?  DNA Sample Prep Kit for Illumina?  SPK0001-V08 N/A
SPARK? DNA 样品预备盒,用于  Ion Torrent? 平台 SPARK?  DNA Sample Prep Kit Ion Torrent? Platform  SPK0002-V08 N/A
核酸10 mM ATP 液 Nucleic  Acid 10 mM ATP Solution N2070-10-L 50  μmol
核酸10 mM dNTP 混合液 Nucleic  Acid 10 mM dNTP Solution Mix N2050-10-L 100  μmol
N2050-10-F 20  μmol
核酸100 mM 75μmol dNTP 混合体系 Nucleic  Acid 100 mM 75μmol dNTP Solution Set N2010L 75  μmol
N2010F 20  μmol
核酸100 mM dATP 液 Nucleic  Acid 100 mM dATP Solution N2010-A-L 75  μmol
核酸25 mM 100?mol dNTP Solution Mix Nucleic  Acid 25 mM 100?mol dNTP Solution Mix N2050L  100  ?mol
N2050F  20  ?mol
大肠杆菌DNA连接酶 E.  coli DNA Ligase  L6090L 2,500  U
T3 DNA连接酶 T3 DNA  Ligase L6010L  900,000  U
L6010F  140,000  U
T4 DNA连接酶 T4  DNA Ligase  L6030-LC-L 150,000  U
T4 DNA连接酶(快速) T4  DNA Ligase (Rapid)  L6030-HC-L 240,000  U
T4 DNA连接酶1 T4 RNA  Ligase 1 L6050L  10,000  U
L6050F  1,540  U
T4 RNA连接酶2 T4  RNA Ligase 2  L6080L 4,500  U
T4 RNA连接酶2截断 T4  RNA Ligase 2 Truncated  L6070L 500  U
T7 DNA连接酶 T7 DNA  Ligase L6020L  900,000  U
L6020F  138,000  U
Taq DNA连接酶 Taq  DNA Ligase L6060L  20,000  U
L6060F  3,100  U
 DNA聚合酶I  DNA  Polymerase I  P7050L 5,000  U
Klenow (3′→ 5′ exo-)(高浓度) Klenow  (3′→ 5′ exo-) (High Concentration)  P7010-HC-L 10,000  U
P7010-LC-L 10,000  U
Klenow片段 Klenow  Fragment  P7060L 2,500  U
M-MuLV 逆转录酶 M-MuLV  Reverse Transcriptase P7040L  100,000  U
P7040F  15,400  U
Mako DNA 聚合酶 (3′→ 5′ exo-) Mako  DNA Polymerase (3′→ 5′ exo-)  P7090L 3,000  U
Manta 1.0 DNA 聚合酶(高浓度) Manta  1.0 DNA Polymerase (High Concentration) P7140-HC-L 100,000  U

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