请使用支持JavaScript的浏览器!
主营:连接蛋白、DNA修饰酶、NGS文库制备(NGS Library Construction)、核酸和RNA酶。
banner
当前位置: 首页 > 产品中心 > Ultrapure Ligases > Enzymatics/T4 RNA Ligase 2 Truncated/L6070L/500 U
公司介绍
Enzymatics is an emerging leader in the supply of the enzymes that drive nucleic acid detection technologies. Our leadership is currently building a team which embodies our mission to lead the molecular biology tools market in quality, customer servi...

Enzymatics/T4 RNA Ligase 2 Truncated/L6070L/500 U

Enzymatics/T4 RNA Ligase 2 Truncated/L6070L/500 U
  • Enzymatics/T4 RNA Ligase 2 Truncated/L6070L/500 U
商品介绍

T4RNALigase2Truncated

ProductDescription

T4RNALigase2truncatedcatalyzesphosphodiesterbondformationbetweenapre-adenylated5′phosphate(DNAorRNA)andthe3′hydroxylofRNA.Thetruncatedenzymecontainsthefirst249aminoacidswhichmakestheenzymerequireapre-adenylated5′terminaldonorandeliminatestheneedforATP.BecauseT4RNAligase2truncatedcannotusethe5′phosphateofRNAorDNAasadonorintheligationreaction,itisusefulforcertainapplicationssuchaslinkerligationswithpre-adenylated5′DNAto3′hydroxylRNA.Thedesiredspecificligationproductsareenhanceddramaticallyoverunwantedbackgroundligationproducts,makingthetruncatedenzymesuperiortothefull-lengthenzymeforthisuse.

SourceofProtein
Purifiedfromastrainof E.coli thatexpressestherecombinanttruncatedT4RNALigase2gene.

Suppliedin
10mMTris-HCl
100mMNaCl
0.1mMDTT
0.1mMEDTA
50%glycerol
pH7.5@25°C

SuppliedWith:
B607010XT4RNALigase2,TruncatedBuffer

10XT4RNALigase2,TruncatedBuffer(B6070)
500mMTris-HCl
100mMMgCl2
50mMDTT
pH7.6@25°C

UnitDefinition
Oneunitisdefinedastheamountofenzymerequiredtoligate50%of0.4µgofanequimolarmixofasingle-stranded5′FAM-labeled17-merRNAtothe5′pre-adenylatedendofa18-merDNAwhenboth17-mersareannealedtoacomplementary35-merDNAstrandin20µL1Xreactionbufferfollowinga30minuteincubationat37°C.

QualityControlAnalysis

UnitCharacterizationAssay
Specificactivitywasmeasuredusinga2-foldserialdilutionmethod.Dilutionsofenzymeweremadein1Xreactionbufferand2µLofeachenzymedilutionwasaddedto18µLreactionsin1Xreactionbuffercontaining0.4µgofanequimolarmixofone17baseRNAoligonucleotide(5′FAM-labeled)andone18baseDNAoligonucleotide(5′pre-adenylated)annealedtoacomplementary35-merDNAoligonucleotide.Reactionswereincubated30minutesat37°C,quenched,andanalyzedona15%TBE-Ureagel.

ProteinConcentration(OD280)Measurement
TheenzymewasanalyzedatOD280 usingaNanodropspectrophotometerstandardizedwitha2.0mg/mlBSAsampleandblankedwithproductstoragesolution.Theobservedaveragemeasurementof3replicatesampleswasconvertedtomg/mLusinganextinctioncoefficientof33,710andmolecularweightof30,451Daltons.

SDS-Page(PhysicalPurityAssessment)
Aconcentratedsampleofenzymewasloadedonadenaturing4-20%Tris-GlycineSDS-PAGEgelflankedbyabroad-rangeMWMarkeranda1:100dilutionofthesample.Followingelectrophoresis,thegelwasstainedandthesamplescomparedtodeterminephysicalpurity.Theacceptancecriteriaforthistestrequiresthattheaggregatemassofcontaminantbandsintheconcentratedsampledonotexceedthemassoftheproteinofinterestbandinthedilutesample.Comparisonbetweentheconcentratedandthedilutedsamplesisusedtoevaluatepercentpurity.

ContaminationTests

Single-StrandedExonucleaseActivity
RADIolabeledsingle-strandedDNAsubstrateincubatedwithenzymesolutionfor4hoursat37°Cresultedinlessthan5%releaseofTCA-solublecounts.

Double-StrandedExonucleaseActivity
Radiolabeleddouble-strandedDNAsubstrateincubatedwithenzymesolutionfor4hoursat37°Cresultedinlessthan1%releaseofTCA-solublecounts.

Double-StrandedEndonucleaseActivity
AreactioncontainingplasmidDNAincubatedwithenzymesolutionfor4hoursat37°CresultedinnovisuallydiscernIBLeconversiontonickedcircularDNAasdeterminedbyagarosegelelectrophoresis.

E.coli 16SrDNAContaminationTest
ReplicatesamplesofenzymeweredenaturedandscreenedinaTaqManqPCRassayforthepresenceofcontaminatingE.coli genomicDNAusingoligonucleotideprimerscorrespondingtothe16SrRNAlocus.Theacceptancecriterionforthetestisthethresholdcyclecount(Ct)producedbytheaverageof3replicatenotemplatecontrolsamples.BasedonthecorrelationbetweenthenotemplatecontrolCt values,andstandardcurvedata,thedetectionlimitofthisassayis<10copiesgenome/sample.

Non-SpecificRNAseAssay
Theenzymewasscreenedfornon-specificRNAsecontaminationusingtheRNAseAlertkit,(IntegratedDNATechnologies),followingthemanufacturer’sguidelines.

ProductInformation

T4RNALigase2Truncated
PartNumberL6070L
Price$363
Concentration5,000U/ml
UnitSize500U
SDSAvailableonrequest

ProductSpecification*

StorageTemperature-25to-15°C
TestSpecification
Purity(SDS-PAGE)>99%
SpecificActivity30,000U/mg
SSExonuclease50U<5%released
DSExonuclease50U<1%released
DSEndonuclease50U=Noconversion
E.coliDNAContamination50U<10copies
RNAseContamination50U=Nodetectablenon-specificRNAse

*Foradetailedsummaryofassayconditionsanddata,refertotheQualityControlsAnalysissection.

Notes

Enzymatics’T4RNALigase2Truncateddemonstratesequivalentorsuperiorvolume/volumeperformancewhencomparedtoanalogousT4RNALigase2Truncatedproductsonthemarket.

References

  1. Ho,C.K.etal.(2004)Structure,12,327-339.
  2. Ho,C.K.andShuman,S.(2002)Proc.Natl.Acad.Sci.USA,99,12709-12714.
  3. Nandakumar,J.etal.(2004)J.Biol.Chem,279,31337-31347.
  4. Aravin,A.andTusch,T.(2005)FEBSLetters,579,5830-5840.
  5. Pfeffer,S.etal.(2005)Nat.Meth,2,269-276.

LimitationsofUse
Thisproductwasdeveloped,manufactured,andsoldforinvitrouseonly.TheproductisnotsuitableforadmiNISTrationtohumansoranimals.SDSsheetsrelevanttothisproductareavailableuponrequest.

品牌介绍

Enzymatics is an emerging leader in the supply of the enzymes that drive nucleic acid detection technologies. Our leadership is currently building a team which embodies our mission to lead the molecular biology tools market in quality, customer service, and innovation. We seek engaging, motivated individuals who can articulate their goals and, in turn, offer a culture that breeds success. Enzymatics is an employee-focused organization that strives to cultivate challenging careers and rewarding lives. If you seek a challenge, have a thirst for knowledge, and are interested in having fun while working, please apply to learn more!

We offer a competitive benefits package and an incentive-based compensation package.



苏州蚂蚁淘生物科技有限公司是一家创新型高科技生物公司,以常规生物学实验外包与生物学试剂为基础,放眼于生命科学领域的前沿技术服务,公司技术骨干有着多年的生物学科研背景,毕业于国内外名牌大学,并且有着丰富的项目经验,了解国内外科研行情,我公司力求为每一个客户提专业、针对性的实验方案。我公司以技术服务为支点,和国内外多家生物试剂公司有着密切的业务往来.公司特色技术服务包括:抗体药物研发、噬菌体抗体库、蛋白表达与传化、抗体制备、高通量测序、组学研究、生物信息学分析与方法建立、基础分子生物学技术外包、化学中间体合成、原料药物研发、论文编写等。我公司秉承”诚信做人,认真做事,服务至上“,希望用我们的专业知识和项目经验为国内科研客户解决科研问题,祝您科研之路畅通无阻!


世界*实验材料供应商 Enzymatics正式授权苏州蚂蚁淘为其中国代理, Enzymatics在一直是行业的标杆,一直为广大科研客户提供zui为的产品和服务,苏州蚂蚁淘一直秉承为中国科研客户带来的产品,的服务,签约 Enzymatics就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

Enzymatics中国代理, Enzymatics上海代理, Enzymatics北京代理,Enzymatics广东代理, Enzymatics江苏代理Enzymatics湖北代理,Enzymatics天津,Enzymatics黑龙江代理,Enzymatics内蒙古代理,Enzymatics吉林代理,Enzymatics福建代理, Enzymatics江苏代理, Enzymatics浙江代理, Enzymatics四川代理,

 

酶产品在生命科学研究中是不可或缺的重要工具,在科学研究中对于其性能、纯度和稳定性的要求日益提升。基因公司*代理的酶产品专家—Enzymatics一贯为客户提供zui高质量和zui优价值的产品,因此我们竭诚向您推荐 Enzymatics产品。

Enzymatics公司建立于2006年,专门从事开发、制造和分销酶产品,致力于酶产品研发,为市场带来了特殊的、高品质的产品。 Enzymatics公司建立了严格完整的操作体系,通过了ISO13485:2003和9001:2000体系标准认证,并符合FDA 21 CFR.820质量系统法规中对于常规用途试剂的生产规范。同时Enzymatics的生产能力被许多FDA认证的医疗设备制造商以及国际的ISO认证机构TüV SüD 认可。

自营商城图标
厂家直采
全球直采模式 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔
在线客服
客服电话

4000-520-616

0512-67156496