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主营:连接蛋白、DNA修饰酶、NGS文库制备(NGS Library Construction)、核酸和RNA酶。
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公司介绍
Enzymatics is an emerging leader in the supply of the enzymes that drive nucleic acid detection technologies. Our leadership is currently building a team which embodies our mission to lead the molecular biology tools market in quality, customer servi...

Enzymatics/T4 RNA Ligase 1/L6050L/10,000 U

Enzymatics/T4 RNA Ligase 1/L6050L/10,000 U
  • Enzymatics/T4 RNA Ligase 1/L6050L/10,000 U
商品介绍

T4RNALigase1

ProductDescription

T4RNALigasecatalyzestheATP-dependentligationofsingle-strandednucleicacids(RNAorDNA).

SourceofProtein
Arecombinant E.coli straincarryingtheT4RNALigasegenefrombacteriophageT4.

Suppliedin
10mMTris-HCl
50mMKCl
0.1mMEDTA
1.0mMDTT
50%glycerol
pH7.5@25°C

SuppliedWith 
B6050(10XT4RNALigaseBuffer)

10XT4RNALigaseBuffer(B6050)
500mMTris-HCl
100mMMgCl2
10mMATP
100mMDTT
pH7.8@25°C

UnitDefinition
Oneunitisdefinedastheamountofenzymerequiredtoligate50%of0.4µgofanequimolarmixoftwosinglestranded23baseRNAoligonucleotides(one5′-phosphorylated)in20µL1XT4RNALigaseBufferfollowinga30minuteincubationat37°C.

 

QualityControlAnalysis

UnitCharacterizationAssay
Specificactivitywasmeasuredusinga2-foldserialdilutionmethod.Dilutionsofenzymeweremadein1XT4RNALigasereactionbufferandaddedto20µLreactionscontaining0.4µgofanequimolarmixoftwosingle-stranded23baseRNAoligonucleotides(one5′-phosphorylated)and1XT4RNALigaseBuffer.Reactionswereincubated30minutesat37°C,stopped,andanalyzedona15%TBE-UreagelstainedwithSYBR®GoldNucleicAcidGelStain(InvitrogenS-11494).

ProteinConcentration(OD280)Measurement
A2.0µLsampleofenzymewasanalyzedatOD280 usingaNanodropND-1000spectrophotometerstandardizedusinga2.0mg/mlBSAsample(PierceCat#23209)andblankedwithproductstoragesolution.Theobservedaveragemeasurementof3replicatesampleswasconvertedtomg/mLusinganextinctioncoefficientof49,070andmolecularweightof43,509Daltons.

SDS-Page(PhysicalPurityAssessment)
2.0µLofconcentratedenzymesolutionwasloadedonadenaturing4-20%Tris-GlycineSDS-PAGEgelflankedbyabroad-rangeMWMarkerand2.0µLofa1:100dilutionofthesample.Followingelectrophoresis,thegelwasstainedandthesamplescomparedtodeterminephysicalpurity.Theacceptancecriteriaforthistestrequiresthattheaggregatemassofcontaminantbandsintheconcentratedsampledonotexceedthemassoftheproteinofinterestbandinthedilutesample,confirminggreaterthan99%purityoftheconcentratedsample.

ContaminationTests

Single-StrandedExonucleaseActivity
A50µlreactioncontaining10,000cpmofarADIolabeledsingle-strandedDNAsubstrateand10µLofenzymesolutionincubatedfor4hoursat37°Cresultedinlessthan5.0%releaseofTCA-solublecounts.

Double-StrandedExonucleaseActivity
A50µlreactioncontaining5,000cpmofaradiolabeleddouble-strandedDNAsubstrateand10µLofenzymesolutionincubatedfor4hoursat37°Cresultedinlessthan1.0%releaseofTCA-solublecounts.

Double-StrandedEndonucleaseActivity
A50µLreactioncontaining0.5µgofpBR322DNAand10µLofenzymesolutionincubatedfor4hoursat37°CresultedinnovisuallydiscernIBLeconversiontonickedcircularDNAasdeterminedbyagarosegelelectrophoresis.

E.coli 16SrDNAContaminationTest
Replicate5µLsamplesofenzymesolutionweredenaturedandscreenedinaTaqManqPCRassayforthepresenceofcontaminating E.coli genomicDNAusingoligonucleotideprimerscorrespondingtothe16SrRNAlocus.Theacceptancecriterionforthetestisthethresholdcyclecount(Ct)producedbytheaverageof3replicatenotemplatecontrolsamples.BasedonthecorrelationbetweenthenotemplatecontrolCt values,andstandardcurvedata,thedetectionlimitofthisassayis<10copiesgenome/sample.

Non-SpecificRNAseAssay
Productwasscreenedfornon-specificRNAsecontaminationusingtheRNAseAlertkit,(IntegratedDNATechnologies),followingthemanufacturer’sguidelines.

ProductInformation

T4RNALigase1
PartNumberL6050L
Price$363
Concentration20,000U/ml
UnitSize10,000U
SDSAvailableonrequest

ProductSpecification*

StorageTemperature-25to-15°C
TestSpecification
Purity(SDS-PAGE)>99%
SpecificActivity16,800U/mg
SSExonuclease200U<5.0%released
DSExonuclease200U<1.0%released
DSEndonuclease200U=Noconversion
E.coliDNAContamination200U<10copies
Non-specificRNAse200Unonedetected

*Foradetailedsummaryofassayconditionsanddata,refertotheQualityControlsAnalysissection.

LimitationsofUse
Thisproductwasdeveloped,manufactured,andsoldforinvitrouseonly.TheproductisnotsuitableforadmiNISTrationtohumansoranimals.SDSsheetsrelevanttothisproductareavailableuponrequest.

品牌介绍

Enzymatics is an emerging leader in the supply of the enzymes that drive nucleic acid detection technologies. Our leadership is currently building a team which embodies our mission to lead the molecular biology tools market in quality, customer service, and innovation. We seek engaging, motivated individuals who can articulate their goals and, in turn, offer a culture that breeds success. Enzymatics is an employee-focused organization that strives to cultivate challenging careers and rewarding lives. If you seek a challenge, have a thirst for knowledge, and are interested in having fun while working, please apply to learn more!

We offer a competitive benefits package and an incentive-based compensation package.



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恭喜蚂蚁淘获得Enzymatics公司代理授权

蚂蚁淘(ebiomall)是一家生命科学领域的垂直电商平台,公司专注生物医学科研用品的 全球导购和品牌推广,为海外厂方与国内代理经销商搭建跨境贸易系统和交流平台。

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2019年蚂蚁淘再获得英国TwistDx公司代理授权

订货请咨询苏州蚂蚁淘生物科技有限公司

关于Enzymatics

Qiagen旗下的Enzymatics品牌专注于酶产品的研发,其生产的高品质酶类产品促进了核酸检测技术的发展,使全球客户变革了诊断、治疗和生命科学的研究。业务范围包括:试剂、供应链解决方案,应用科学等。其测序产品市场占有率高达80%。

产品:

超纯连接酶(如T4连接酶)、超纯poly酶、Archer™系列测序工具(Archer Products)、连接蛋白、DNA修饰酶、NGS文库制备(NGS Library Construction)、核酸和RNA酶。满足用户丰富多样的研究需求。

应用:

Enzymatics的酶产品被广泛应用于生命科学、临床应用领域二代测序和其他遗传分析技术领域。

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