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主营:连接蛋白、DNA修饰酶、NGS文库制备(NGS Library Construction)、核酸和RNA酶。
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公司介绍
Enzymatics is an emerging leader in the supply of the enzymes that drive nucleic acid detection technologies. Our leadership is currently building a team which embodies our mission to lead the molecular biology tools market in quality, customer servi...

Enzymatics/RNAse Inhibitor/Y9240L/20,000 U

Enzymatics/RNAse Inhibitor/Y9240L/20,000 U
  • Enzymatics/RNAse Inhibitor/Y9240L/20,000 U
商品介绍

RNAseInhibitor

ProductDescription

RNAseInhibitorisanacidic,52kDaproteinthatisapotentnon-competitiveinhibitorofpancreatic-typeribonucleasessuchasRNaseA,RNaseB,andRNaseC.TheenzymeisprovidedasafusionoftheporcineRNAseInhibitorgenewithaproprietary,22.5kDaproteintag.

SourceofProtein
ArecombinantE.colistraincarryingtheporcineRNAseInhibitorgene.

Suppliedin
20mMHepes-KOH
50mMKCl
8mMDTT
50%glycerol
pH7.5@25°C

UnitDefinition
Oneunitisdefinedastheamountofenzymerequiredtoinhibitby50%thehydrolysisofcytidine2′,3′-cyclicmonophosphateby5ngofRNAseA.(1)

QualityControlAnalysis

UnitCharacterizationAssay
Specificactivitywasdeterminedusing1.1-foldserialdilutionmethod.Dilutionsofenzymeweremadein1XRNAseInhibitorReactionBufferandaddedto1000µLreactionscontaining1mMcytidine2′,3′-cyclicmonophosphate,1µgRNaseAina1Xreactionbuffercontaining100mMTris-Acetate,1mMEDTA,pH6.5.Absorbanceat286nmwasobservedat20secondintervalsduringa5minutereaction.

ProteinConcentration(OD280)Measurement
A2.0µLsampleofenzymewasanalyzedatOD280usingaNanodropND-1000spectrophotometerstandardizedusinga2.0mg/mlBSAsample(PierceCat#23209)andblankedwithproductstoragesolution.Theobservedaveragemeasurementof3replicatesampleswasconvertedtomg/mLusinganextinctioncoefficientof59,600andmolecularweightof74,828Daltons.

SDS-Page(PhysicalPurityAssessment)
2.0µLofconcentratedenzymesolutionwasloadedonadenaturing4-20%Tris-GlycineSDS-PAGEgelflankedbyabroad-rangeMWMarkerand2.0µLofa1:100dilutionofthesample.Followingelectrophoresis,thegelwasstainedandthesamplescomparedtodeterminephysicalpurity.Theacceptancecriteriaforthistestrequiresthattheaggregatemassofcontaminantbandsintheconcentratedsampledonotexceedthemassoftheproteinofinterestbandinthedilutesample,confirminggreaterthan99%purityoftheconcentratedsample.

ContaminationTests

Single-StrandedExonucleaseActivity
A50µlreactioncontaining10,000cpmofarADIolabeledsingle-strandedDNAsubstrateand10µLofenzymesolutionincubatedfor4hoursat37°Cresultedinlessthan5.0%releaseofTCA-solublecounts.

Double-StrandedExonucleaseActivity
A50µlreactioncontaining5,000cpmofaradiolabeleddouble-strandedDNAsubstrateand10µLofenzymesolutionincubatedfor4hoursat37°Cresultedinlessthan1.0%releaseofTCA-solublecounts.

Double-StrandedEndonucleaseActivity
A50µLreactioncontaining0.5µgofpBR322DNAand10µLofenzymesolutionincubatedfor4hoursat37°CresultedinnovisuallydiscernIBLeconversiontonickedcircularDNAasdeterminedbyagarosegelelectrophoresis.

Non-SpecificRNAseAssay
Productwasscreenedfornon-specificRNAsecontaminationusingtheRNAseAlertkit,(IntegratedDNATechnologies),followingthemanufacturer’sguidelines.

E.coli16SrDNAContaminationTest
Replicate5µLsamplesofenzymesolutionweredenaturedandscreenedinaTaqManqPCRassayforthepresenceofcontaminatingE.coligenomicDNAusingoligonucleotideprimerscorrespondingtothe16SrRNAlocus.Theacceptancecriterionforthetestisthethresholdcyclecount(Ct)producedbytheaverageof3replicatenotemplatecontrolsamples.BasedonthecorrelationbetweenthenotemplatecontrolCtvalues,andstandardcurvedata,thedetectionlimitofthisassayis<10copiesgenome/sample.

ProductInformation

RNAseInhibitor
PartNumberY9240L
Price$363
Concentration40,000U/ml
UnitSize20,000U
SDSAvailableonrequest

ProductSpecification*

StorageTemperature-25to-15°C
TestSpecification
Purity(SDS-PAGE)>99%
SSExonuclease2000U<5.0%released
DSExonuclease2000U<1.0%released
DSEndonuclease2000U=Noconversion
E.coliDNAContamination2000U<10copies
RNAseContamination2,000U=NoDetectablenon-specificRNase

*Foradetailedsummaryofassayconditionsanddata,refertotheQualityControlsAnalysissection.

References

  1. Blackburn,P.,1979.RibonulceaseInhibitorfromHumanPlacenta:RapidPurificationandAssay.TheJournalofBIOLOGicalChemistry,Vol.254,No.24pp12484-12487.

LimitationsofUse
Thisproductwasdeveloped,manufactured,andsoldforinvitrouseonly.TheproductisnotsuitableforadmiNISTrationtohumansoranimals.SDSsheetsrelevanttothisproductareavailableuponrequest.

品牌介绍

Enzymatics is an emerging leader in the supply of the enzymes that drive nucleic acid detection technologies. Our leadership is currently building a team which embodies our mission to lead the molecular biology tools market in quality, customer service, and innovation. We seek engaging, motivated individuals who can articulate their goals and, in turn, offer a culture that breeds success. Enzymatics is an employee-focused organization that strives to cultivate challenging careers and rewarding lives. If you seek a challenge, have a thirst for knowledge, and are interested in having fun while working, please apply to learn more!

We offer a competitive benefits package and an incentive-based compensation package.



苏州蚂蚁淘生物科技有限公司是一家创新型高科技生物公司,以常规生物学实验外包与生物学试剂为基础,放眼于生命科学领域的前沿技术服务,公司技术骨干有着多年的生物学科研背景,毕业于国内外名牌大学,并且有着丰富的项目经验,了解国内外科研行情,我公司力求为每一个客户提专业、针对性的实验方案。我公司以技术服务为支点,和国内外多家生物试剂公司有着密切的业务往来.公司特色技术服务包括:抗体药物研发、噬菌体抗体库、蛋白表达与传化、抗体制备、高通量测序、组学研究、生物信息学分析与方法建立、基础分子生物学技术外包、化学中间体合成、原料药物研发、论文编写等。我公司秉承”诚信做人,认真做事,服务至上“,希望用我们的专业知识和项目经验为国内科研客户解决科研问题,祝您科研之路畅通无阻!


Enzymatics专注于于酶产品的研发,为市场带来了特殊的、高品质的产品。是一家试剂、试剂盒和测定的领先生产商,使全球客户变革了诊断、治疗和生命科学的研究。业务包括:试剂、供应链解决方案,应用科学。目前市场上80%测序产品来自 Enzymatics(“Today our products underpin over 80% of the sequencing reactions that are run around the globe”)。Enzymatics产品的好处之一--能提高效率:Nature Methods中提到“使用Enzymatics超纯连接酶,连接步骤的效率得以提高,连接片段的产量增加了20-30%”。好处之二--有效降低测序成本:遗传学界的泰斗George Church更是认为Enzymatics是"a powerful catalyst in the democratization of sequencing"。合作伙伴包括:BGI,Berry Genomics ,Illumina,454 Life Sciences和Ion Torrent Syst,皆对Enzymatics的出品赞不绝口。
       Enzymatics产品包括八类:超纯连接酶如T4连接酶、超纯poly酶、ArcherTM系列测序工具、连接蛋白、DNA修饰酶、NGS文库制备、核酸和RNA酶。 Enzymatics公司之操作体系以严格著称,产品通过了ISO13485:2003和9001:2000体系标准认证,并符合FDA 21 CFR.820质量系统法规中对于常规用途试剂的生产规范。与此同时,Enzymatics的生产能力被许多FDA认证的医疗设备制造商以及国际著名的ISO认证机构TÜV SÜD 认可。(http://www.enzymatics.com/)
      目前默瑞生物公司已成功取得Enzymatics中国总代理,Enzymatics各大产品均可在我司查询订购。
 

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