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主营:连接蛋白、DNA修饰酶、NGS文库制备(NGS Library Construction)、核酸和RNA酶。
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公司介绍
Enzymatics is an emerging leader in the supply of the enzymes that drive nucleic acid detection technologies. Our leadership is currently building a team which embodies our mission to lead the molecular biology tools market in quality, customer servi...

Enzymatics/Exonuclease I/X8010L/30,000 U

Enzymatics/Exonuclease I/X8010L/30,000 U
  • Enzymatics/Exonuclease I/X8010L/30,000 U
商品介绍

ExonucleaseI

ProductDescription

ExonucleaseIcleavessingle-strandedDNAinthe3′→5′direction,releasing5′-mono/di-nucleotidesandleavingdouble-strandedDNAmoleculesandthe5′-terminusintact.Theenzymeisprocessivethoughdigestionisinhibitedbythepresenceofa3′-terminalphosphate.ExonucleaseIistolerantofawide-rangeofbufferconditionsandcantypicallybeaddedtoreactionscontainingmagnesium(1-3).

SourceofProtein
Purifiedfromastrainof E.coli thatexpressestherecombinantExonucleaseIgene.

Suppliedin
10 mM Tris-HCl
100 mM NaCl
1 mM DTT
0.5 mM EDTA
50% glycerol
pH7.5 @25°C

UnitDefinition
Oneunitisdefinedastheamountofenzymerequiredtoproduce10nmolofacid-solubletotalnucleotidein30minutesat37°C.

QualityControlAnalysis

UnitCharacterizationAssay
Unitactivitywasmeasuredusinga2-foldserialdilutionmethod.Dilutionsofenzymeweremadeinaglycerol(50%)containingExonucleaseIstoragesolutionandaddedto50µLreactionscontainingasingle-strandedtritiatedDNAfragment,and67mMGlycine-KOH(pH9.5),10mMDTT,6.7mMMgCl2.Reactionswereincubated10minutesat37°C,plungedonice,andanalyzedusingaTCA-precipitationmethod.

ProteinConcentration(OD280)Measurement
A2.0µLsampleofenzymewasanalyzedatOD280 usingaNanodropND-1000spectrophotometerstandardizedusinga2.0mg/mlBSAsample(PierceCat#23209),andblankedwithenzymestoragesolution.Theobservedaveragemeasurementof3replicatesampleswasconvertedtomg/mLusinganextinctioncoefficientof72,970andmolecularweightof54,500Daltons.

SDS-Page(PhysicalPurityAssessment)
2.0µLofconcentratedenzymesolutionwasloadedonadenaturing4-20%Tris-GlycineSDS-PAGEgelflankedbyabroad-rangeMWMarkerand2.0µLofa1:100dilutionofthesample.Followingelectrophoresis,thegelwasstainedandthesamplescomparedtodeterminephysicalpurity.Theacceptancecriteriaforthistestrequiresthattheaggregatemassofcontaminantbandsintheconcentratedsampledonotexceedthemassoftheproteinofinterestbandinthedilutesample,confirminggreaterthan99%purityoftheconcentratedsample.

ContaminationTests

Double-StrandedEndonucleaseActivity
A50µLreactioncontaining0.5µgofpBR322DNAand10µLofenzymesolutionincubatedfor4hoursat37°CresultedinnovisuallydiscernIBLeconversiontonickedcircularDNAasdeterminedbyagarosegelelectrophoresis.

E.coli 16SrDNAContaminationTest
Replicate5µLsamplesofenzymesolutionweredenaturedandscreenedinaTaqManqPCRassayforthepresenceofcontaminating E.coli genomicDNAusingoligonucleotideprimerscorrespondingtothe16SrRNAlocus.Theacceptancecriterionforthetestisthethresholdcyclecount(Ct)producedbytheaverageof3replicatenotemplatecontrolsamples.BasedonthecorrelationbetweenthenotemplatecontrolCt values,andstandardcurvedata,thedetectionlimitofthisassayis<10copiesgenome/sample.

ProductInformation

ExonucleaseI
PartNumberX8010L
Price$363
Concentration20,000U/ml
UnitSize30,000U
SDSAvailableonrequest

ProductSpecification*

StorageTemperature-15to-25°C
TestSpecification
Purity(SDS-PAGE)>99%
SpecificActivity185,000U/mg
DSEndonuclease200U=noconversion
E.coliDNAContamination200U<10copies

*Foradetailedsummaryofassayconditionsanddata,refertotheQualityControlsAnalysissection.

Notes

  1. ExonucleaseIcanbeheat-inactivatedbyincubationat80°Cfor15minutes.
  2. ExonucleaseIwillpreferentiallydegradesingle-strandedoligonucleotideprimersinareactioncontainingamplificationproductsorothersourcesofdouble-strandedDNA,leavingdouble-strandedmoleculesintact.
  3. ExonucleaseI,incombinationwithLamBDaExonuclease(X8030L),iseffectiveinremovinglinearDNAspeciesfromplasmidpreparations.
  4. ExonucleaseIworkswellinmostmolecularBIOLOGybufferswhichcontainmagnesiuminexcessof1.5mM.

References

  1. Lehman,I.R.andNussbaum,A.L.(1964)J.Biol.Chem.239,2628.
  2. Kushner,S.R.etal.(1971)Proc.Natl.Acad.Sci.USA68,824.
  3. Kushner,S.R.etal.(1972)Proc.Natl.Acad.Sci.USA69,1366.

LimitationsofUse
Thisproductwasdeveloped,manufactured,andsoldforinvitrouseonly.TheproductisnotsuitableforadmiNISTrationtohumansoranimals.SDSsheetsrelevanttothisproductareavailableuponrequest.

品牌介绍

Enzymatics is an emerging leader in the supply of the enzymes that drive nucleic acid detection technologies. Our leadership is currently building a team which embodies our mission to lead the molecular biology tools market in quality, customer service, and innovation. We seek engaging, motivated individuals who can articulate their goals and, in turn, offer a culture that breeds success. Enzymatics is an employee-focused organization that strives to cultivate challenging careers and rewarding lives. If you seek a challenge, have a thirst for knowledge, and are interested in having fun while working, please apply to learn more!

We offer a competitive benefits package and an incentive-based compensation package.



苏州蚂蚁淘生物科技有限公司是一家创新型高科技生物公司,以常规生物学实验外包与生物学试剂为基础,放眼于生命科学领域的前沿技术服务,公司技术骨干有着多年的生物学科研背景,毕业于国内外名牌大学,并且有着丰富的项目经验,了解国内外科研行情,我公司力求为每一个客户提专业、针对性的实验方案。我公司以技术服务为支点,和国内外多家生物试剂公司有着密切的业务往来.公司特色技术服务包括:抗体药物研发、噬菌体抗体库、蛋白表达与传化、抗体制备、高通量测序、组学研究、生物信息学分析与方法建立、基础分子生物学技术外包、化学中间体合成、原料药物研发、论文编写等。我公司秉承”诚信做人,认真做事,服务至上“,希望用我们的专业知识和项目经验为国内科研客户解决科研问题,祝您科研之路畅通无阻!


Enzymatics--核酸25 mM 100µmol dNTP Solution Mix--N2050L,N2050F

核酸25mM100µmoldNTPSolutionMix

摘要:四种天然脱氧核苷酸的等摩尔混合溶液(25mM)

产品描述

Anequimolar(25mM)mixtureofthefournaturaldeoxynucleotides.

四种天然脱氧核苷酸的等摩尔混合溶液(25mM)

SuppliedAs:25mMeachdNTP(dATP,dCTP,dGTP,dTTP)atpH7.5

产品信息

PartNumber N2050L,N2050F

Concentration 25mM

UnitSize 100µmol,20µmol

产品特性

StorageTemperature -25to-15°C

Purity ≥99%

BasePurity ≥99.5%

Pyrophosphate ≤0.003pmol/pmolofnucleotide

EnzScript™(MMLV逆转录酶,RNaseH-)

摘要:基于RNA的DNA聚合酶,无核酸内切酶活性

产品描述:

EnzScript™(M-MLV逆转录酶RNaseHminus)属于RNA依赖的DNA聚合酶,该酶无RNaseH活性。EnzScript™被用于从polyAmRNA或总RNA中产生首链cDNA以用于下游的一些用途,如RT-PCR,cDNA克隆或RNA-Seq的文库构建。RNaseH结构域中的点突变能增加酶的热稳定性,相比于野生型M-MLV逆转录酶,它支持全长转录物种产生更高的cDNA产量

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