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公司介绍
Enzymatics is an emerging leader in the supply of the enzymes that drive nucleic acid detection technologies. Our leadership is currently building a team which embodies our mission to lead the molecular biology tools market in quality, customer servi...

Enzymatics/Exonuclease III/X8020L/50,000 U

Enzymatics/Exonuclease III/X8020L/50,000 U
  • Enzymatics/Exonuclease III/X8020L/50,000 U
商品介绍

ExonucleaseIII

ProductDescription

ExonucleaseIIIisa3′→5′exonucleasewhichactsbydigestingonestrandofadsDNAduplexatatimeordigestingtheRNAstrandofanRNA-DNAheteroduplex(1).ExonucleaseIIIbreaksphosphodiesterbondsonthe5′sideofAPsitesinbothdsDNAandssDNA(3),removes3′terminalgroupsondsDNA(3),increasesMutYturnover(4),andefficientlydegrades3′recessedbutnot3′protrudingDNAends(creating5′overhangs)(5).ExoIIIremovesalimitednumberofnucleotidesperbindingevent,resultingincoordinatedprogressivedeletionswithinthepopulationofDNAmolecules(1).

SourceofProtein
Purifiedfromastrainof E.coli thatexpressestherecombinantExonucleaseIIIgene.

Suppliedin
25mMTris-HCl
50mMKCl
1.0mMDTT
0.1%mMEDTA
50%Glycerol
pH8.0@25°C

SuppliedWith
B0130(10XYellowBuffer)

10XYellowBuffer(B0130)
100mMBis-Tris-Propane-HCl
100mMMgCl2
10mMDTT
pH7.0@25°C

UnitDefinition
Oneunitisdefinedastheamountofenzymerequiredtoproduce1nmolofacid-solubletotalnucleotidein30minutesat37°C.

QualityControlAnalysis

UnitCharacterizationAssay
Unitactivitywasmeasuredusinga2-foldserialdilutionmethod.Dilutionsofenzymeweremadein1Xreactionbufferandaddedto50µLreactionscontainingatritiatedDNAfragment,and1XExoIIIYellowBuffer.Reactionswereincubated10minutesat37°C,plungedonice,andanalyzedusingaTCA-precipitationmethod.

ProteinConcentration(OD280)Measurement
A2.0µLsampleofExonucleaseIIIwasanalyzedat 280 usingaNanodropND-1000spectrophotometerstandardizedusinga2.0mg/mlBSAsample(PierceCat#23209),andblankedwithExonucleaseIIIstoragesolution.Theobservedaveragemeasurementof3replicatesampleswasconvertedtomg/mLusinganextinctioncoefficientof38,690andmolecularweightof30,969Daltons.

SDS-Page(PhysicalPurityAssessment)
2.0µLofconcentratedenzymesolutionwasloadedonadenaturing4-20%Tris-GlycineSDS-PAGEgelflankedbyabroad-rangeMWMarkerand2.0µLofa1:100dilutionofthesample.Followingelectrophoresis,thegelwasstainedandthesamplescomparedtodeterminephysicalpurity.Theacceptancecriteriaforthistestrequiresthattheaggregatemassofcontaminantbandsintheconcentratedsampledonotexceedthemassoftheproteinofinterestbandinthedilutesample,confirminggreaterthan99%purityoftheconcentratedsample.

ContaminationTests

Double-StrandedEndonucleaseActivity
A50µLreactioncontaining0.5µgofpBR322DNAand10µLofenzymesolutionincubatedfor4hoursat37°CresultedinnovisuallydiscernIBLeconversiontonickedcircularDNAasdeterminedbyagarosegelelectrophoresis.

E.coli 16SrDNAContaminationTest
Replicate5µLsamplesofenzymesolutionweredenaturedandscreenedinaTaqManqPCRassayforthepresenceofcontaminating E.coli genomicDNAusingoligonucleotideprimerscorrespondingtothe16SrRNAlocus.Theacceptancecriterionforthetestisthethresholdcyclecount(Ct)producedbytheaverageof3replicatenotemplatecontrolsamples.BasedonthecorrelationbetweenthenotemplatecontrolCt values,andstandardcurvedata,thedetectionlimitofthisassayis<10copiesgenome/sample.

UDGContaminationTest
A50µLreactioncontaining1µgoftritiatedUracilcontainingDNAand10µLenzymesolutionincubatedfor40minutesat37°CunderstandardUDGunitcharacterizationconditionsresultedinthemeasurementoflessthan20U/mLUDGactivityasdeterminedbyliquidscintillationanalysis.

ProductInformation

ExonucleaseIII
PartNumberX8020L
Price$363
Concentration100,000U/ml
UnitSize50,000U
SDSAvailableonrequest

ProductSpecification*

StorageTemperature-25to-15°C
TestSpecification
Purity(SDS-PAGE)>99%
SpecificActivity100,000U/mg
DSEndonuclease1000U=Noconversion
E.coliDNAContamination1000U<10copies
UDGActivity<20U/mLactivity

*Foradetailedsummaryofassayconditionsanddata,refertotheQualityControlsAnalysissection.

References

  1. Linn,S.M.(1982)Nucleases,pp.291-309,ColdSpringHarborLaboratoryPress.
  2. Shida,T.,etal.(1996)Nucl.AcidsRes.24(22),4572-4576.
  3. Doetsch,P.W.(1990)Mutat.Res.236(2-3),173-201.
  4. Pope,M.A.,etal.(2002)J.Biol.Chem.277(25),22605-22615.
  5. Henikoff,S.(1984)Gene28,351-359.

LimitationsofUse
Thisproductwasdeveloped,manufactured,andsoldforinvitrouseonly.TheproductisnotsuitableforadmiNISTrationtohumansoranimals.SDSsheetsrelevanttothisproductareavailableuponrequest.

品牌介绍

Enzymatics is an emerging leader in the supply of the enzymes that drive nucleic acid detection technologies. Our leadership is currently building a team which embodies our mission to lead the molecular biology tools market in quality, customer service, and innovation. We seek engaging, motivated individuals who can articulate their goals and, in turn, offer a culture that breeds success. Enzymatics is an employee-focused organization that strives to cultivate challenging careers and rewarding lives. If you seek a challenge, have a thirst for knowledge, and are interested in having fun while working, please apply to learn more!

We offer a competitive benefits package and an incentive-based compensation package.



苏州蚂蚁淘生物科技有限公司是一家创新型高科技生物公司,以常规生物学实验外包与生物学试剂为基础,放眼于生命科学领域的前沿技术服务,公司技术骨干有着多年的生物学科研背景,毕业于国内外名牌大学,并且有着丰富的项目经验,了解国内外科研行情,我公司力求为每一个客户提专业、针对性的实验方案。我公司以技术服务为支点,和国内外多家生物试剂公司有着密切的业务往来.公司特色技术服务包括:抗体药物研发、噬菌体抗体库、蛋白表达与传化、抗体制备、高通量测序、组学研究、生物信息学分析与方法建立、基础分子生物学技术外包、化学中间体合成、原料药物研发、论文编写等。我公司秉承”诚信做人,认真做事,服务至上“,希望用我们的专业知识和项目经验为国内科研客户解决科研问题,祝您科研之路畅通无阻!


Enzymatics是分子生物学试剂和制造服务的L先供应商,提供了无与伦比的质量,*性和价值的商业基因组科学界。该公司在美国制造商在ISO 13485认证,专注于建立长期合作伙伴关系,并利用内部和外部创新的突破性技术商业化。

详细介绍


Product Name Part No. Unit Size Concentration Notes
NEW PRODUCTS:
E. coli DNA Ligase L6090L 2,500 10,000 U/ml Efficiently ligates DNA at nicks and cohesive termini. Blunt-ended termini can be joined in the presence of PEG
T4 RNA Ligase 2 L6080L 4,500 30,000 U/ml Ligates nicks on double stranded DNA and from the 3′ OH of RNA to the 5′ phosphate of DNA in double stranded structures
T4 RNA Ligase 2 Truncated L6070L 500 5,000 U/ml Requires a preadenylated 5′ phosphate containing DNA or RNA to ligate to 3′ hydroxyl of RNA
Omni Klentaq® P7500-HC-L 8,400 42,000 U/ml Engineered Taq Polymerase mutant which enables PCR direct from blood, soil, water and food samples. Increased tolerance to fluorescent intercalating dyes
(High Concentration)
Omni Klentaq® P7500-LC-L 8,400 4,200 U/ml Lower concentration – 1 microliter per reaction
(Low Concentration)
Phoenix Hot Start Taq DNA Polymerase P7590L 500 5,000 U/ml Antibody based Hot Start Taq DNA polymerase delivers robust PCR performance with exceptional pre-PCR cycling room temperature stability
VeraSeq™ 2.0 High-Fidelity DNA Polymerase P7511L 500 2,000 U/ml Industry-leading speed, fidelity and robustness delivered via an engineered PCR polymerase
VeraSeq™ ULtra DNA Polymerase P7520L 500 2,000 U/ml High fidelity, speed, and robustness in PCR delivered by an engineered, thermostable DNA polymerase that tolerates uracil templates, nucleotides and primers
RecA Y9260L 1.5 mg 2.0 mg/ml Promotes strand exchange of single-stranded DNA fragments with DNA duplex substrates
Thermolabile Phosphatase Y9210L 5,500 5,000 U/ml Removes 5′ and 3′-phosphate groups from DNA
Thermolabile UDG G5020L 500 1,000 U/ml Removes uracil from DNA, leaving AP site
Poly A Polymerase P7460L 1,000 5,000 U/ml Catalyzes the addition of AMP from ATP to the 3′ hydroxyl of RNA which is useful for making polyA tailed RNA
Ultra Pure Ligases:
Bitmap E. coli DNA Ligase L6090L 2,500 10,000 U/ml Efficiently ligates DNA at nicks and cohesive termini. Blunt-ended termini can be joined in the presence of PEG
T3 DNA Ligase L6010L 900,000 3,000,000 U/ml Works in high ionic strength – to 1.0 M NaCl
T4 DNA Ligase L6030-LC-L 150,000 120,000 U/ml Low concentration overnight ligation format
T4 DNA Ligase (Rapid) L6030-HC-L 240,000 600,000 U/ml “Ultrapure ligase” referenced in Nature Methods (Quail, 1 December 2008)
Bitmap T4 RNA Ligase 1 L6050L 10,000 20,000 U/ml Single-stranded RNA ligase. Also joins single-stranded DNA molecules
T4 RNA Ligase 2 L6080L 4,500 30,000 U/ml Ligates nicks on double stranded DNA and from the 3′ OH of RNA to the 5′ phosphate of DNA in double stranded structures
Bitmap T4 RNA Ligase 2 Truncated L6070L 500 5,000 U/ml Requires a preadenylated 5′ phosphate containing DNA or RNA to ligate to 3′ hydroxyl of RNA
T7 DNA Ligase L6020L 900,000 3,000,000 U/ml 1000 fold higher activity on sticky ends than blunt ends
Taq DNA Ligase L6060L 20,000 40,000 U/ml Thermostable DNA ligase which efficiently seals nicks and discriminates against mismatch ligation
Ultra Pure Polymerases:
DNA Polymerase I P7050L 5,000 10,000 U/ml DNA polymerase with 5′→ 3′ synthesis,
5′→ 3′ and 3′→ 5′ exonuclease activities
Klenow (3′→ 5′ exo-) P7010-HC-L 10,000 50,000 U/ml Proven choice for DNA labeling, A-tailing
Klenow (3′→ 5′ exo-) P7010-LC-L 10,000 5,000 U/ml Proven choice for DNA labeling, A-tailing
Klenow Fragment P7060L 2,500 5,000 U/ml DNA polymerase useful for end-filling prior to blunt-end ligation
Mako DNA Polymerase P7090L 3,000 30,000 U/ml Exonuclease-deficient polymerase, lacks strand displacement activity
Manta 1.0 DNA Polymerase P7140-HC-L 100,000 400,000 U/ml Thermostable Bacillus DNA Polymerase, strong strand displacement
(High Concentration)
Manta 1.0 DNA Polymerase P7140-LC-L 100,000 40,000 U/ml Thermostable Bacillus DNA Polymerase, strong strand displacement
(Low Concentration)
Bitmap M MuLV Reverse Transcriptase P7040L 100,000 200,000 U/ml Useful polymerase for first-strand DNA synthesis
Omni Klentaq® P7500-HC-L 8,400 42,000 U/ml Engineered Taq Polymerase mutant which enables PCR direct from blood, soil, water and food samples. Increased tolerance to fluorescent intercalating dyes
Bitmap (High Concentration)
Omni Klentaq® P7500-LC-L 8,400 4,200 U/ml Lower concentration – 1 microliter per reaction
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