请使用支持JavaScript的浏览器!
主营:连接蛋白、DNA修饰酶、NGS文库制备(NGS Library Construction)、核酸和RNA酶。
banner
当前位置: 首页 > 产品中心 > Binding Proteins > Enzymatics/E. coli Single-Stranded DNA Binding Protein/Y9030L/1.5 mg
公司介绍
Enzymatics is an emerging leader in the supply of the enzymes that drive nucleic acid detection technologies. Our leadership is currently building a team which embodies our mission to lead the molecular biology tools market in quality, customer servi...

Enzymatics/E. coli Single-Stranded DNA Binding Protein/Y9030L/1.5 mg

Enzymatics/E. coli Single-Stranded DNA Binding Protein/Y9030L/1.5 mg
  • Enzymatics/E. coli Single-Stranded DNA Binding Protein/Y9030L/1.5 mg
商品介绍

E.coliSingle-StrandedDNABindingProtein

ProductDescription

Single-StrandedDNABindingProtein(SSB)preferentiallybindssingle-strandedDNA,formingatetrameroffouridentical18.9kDasubunitswhichprotects8-16nucleotides,whilenotbindingwelltodouble-strandedDNA.Innature,SSBparticipatesinDNAreplication,recombination,andrepairfunctions. Invitro,SSBhasbeenfoundtostimulatecertainDNApolymerase-mediatedreactionsbyrelaxingDNAsecondarystructureandenhancingenzymeprocessivity.

SourceofProtein
Arecombinant E.coli straincarryingthe E.coli SSBgene.

Suppliedin
50mMTris-HCl
200mMNaCl
1.0mMDTT
0.1mMEDTA
50%glycerol
pH7.5@25°C

QualityControlAnalysis

ProteinConcentration(OD280)Measurement
A2.0µLsampleofenzymewasanalyzedatOD280 usingaNanodropND-1000spectrophotometerstandardizedusinga2.0mg/mlBSAsample(PierceCat#23209)andblankedwithproductstoragesolution.Theobservedaveragemeasurementof3replicatesampleswasconvertedtomg/mLusinganextinctioncoefficientof27,880andmolecularweightof18,975Daltons.

SDS-Page(PhysicalPurityAssessment)
2.0µLofconcentratedenzymesolutionwasloadedonadenaturing4-20%Tris-GlycineSDS-PAGEgelflankedbyabroad-rangeMWMarkerand2.0µLofa1:100dilutionofthesample.Followingelectrophoresis,thegelwasstainedandthesamplescomparedtodeterminephysicalpurity.Theacceptancecriteriaforthistestrequiresthattheaggregatemassofcontaminantbandsintheconcentratedsampledonotexceedthemassoftheproteinofinterestbandinthedilutesample,confirminggreaterthan99%purityoftheconcentratedsample.

ContaminationTests

Single-StrandedExonucleaseActivity
A50µLreactioncontaining10,000cpmofarADIolabeledsingle-strandedDNAsubstrateand10µµLofenzymesolutionincubatedfor4hoursat37°Cresultedinlessthan1.0%releaseofTCA-solublecounts.

Double-StrandedExonucleaseActivity
A50µLreactioncontaining5,000cpmofaradiolabeleddouble-strandedDNAsubstrateand10µLofenzymesolutionincubatedfor4hoursat37°Cresultedinlessthan1.0%releaseofTCA-solublecounts.

Double-StrandedEndonucleaseActivity
A50µLreactioncontaining0.5µgofpBR322DNAand10µLofenzymesolutionincubatedfor4hoursat37°CresultedinnovisuallydiscernIBLeconversiontonickedcircularDNAasdeterminedbyagarosegelelectrophoresis.

DNABindingAssay
Single-strandedDNAbindingABIlitywasconfirmedinaPCRinhibitionassaybyaddingdecreasingamountsofE.coliSSBtoaseriesofPCRreactionscontainingtargetDNA,200µMdNTPs,1XPCRbufferandTaqDNAPolymerase.Reactionswereincubatedinathermalcyclerandsubjectedto25PCRcycles.SampleswereresolvedusingagarosegelelectrophoresisandamountofSSBrequiredtoblock100%accumulationofPCRproductwasrecorded.Acceptancecriteriaforassay:0.70µgE.coliSSBisrequiredtoinhibitPCRamplificationof5ngtargetDNAfollowing25cyclesofPCR.

ProductInformation

E.coliSingle-StrandedDNABindingProtein
PartNumberY9030L
Price$363
Concentration2.75mg/ml
UnitSize1.5mg
SDSAvailableonrequest

ProductSpecification*

StorageTemperature-25to-15°C
TestSpecification
Purity(SDS-PAGE)>99%
SSExonuclease25µg<1.0%release
DSExonuclease25µg<1.0%release
DSEndonuclease25µg=Noconversion
DNABindingAssay0.7µginhibitsPCR

*Foradetailedsummaryofassayconditionsanddata,refertotheQualityControlsAnalysissection.

LimitationsofUse
Thisproductwasdeveloped,manufactured,andsoldforinvitrouseonly.TheproductisnotsuitableforadmiNISTrationtohumansoranimals.SDSsheetsrelevanttothisproductareavailableuponrequest.

品牌介绍

Enzymatics is an emerging leader in the supply of the enzymes that drive nucleic acid detection technologies. Our leadership is currently building a team which embodies our mission to lead the molecular biology tools market in quality, customer service, and innovation. We seek engaging, motivated individuals who can articulate their goals and, in turn, offer a culture that breeds success. Enzymatics is an employee-focused organization that strives to cultivate challenging careers and rewarding lives. If you seek a challenge, have a thirst for knowledge, and are interested in having fun while working, please apply to learn more!

We offer a competitive benefits package and an incentive-based compensation package.



苏州蚂蚁淘生物科技有限公司是一家创新型高科技生物公司,以常规生物学实验外包与生物学试剂为基础,放眼于生命科学领域的前沿技术服务,公司技术骨干有着多年的生物学科研背景,毕业于国内外名牌大学,并且有着丰富的项目经验,了解国内外科研行情,我公司力求为每一个客户提专业、针对性的实验方案。我公司以技术服务为支点,和国内外多家生物试剂公司有着密切的业务往来.公司特色技术服务包括:抗体药物研发、噬菌体抗体库、蛋白表达与传化、抗体制备、高通量测序、组学研究、生物信息学分析与方法建立、基础分子生物学技术外包、化学中间体合成、原料药物研发、论文编写等。我公司秉承”诚信做人,认真做事,服务至上“,希望用我们的专业知识和项目经验为国内科研客户解决科研问题,祝您科研之路畅通无阻!


〖招商品牌之enzymatics酶科技〗

 

蚂蚁淘生物独家代理的酶产品专家--enzymatics,(http://www.enzymatics.com/),于2015年1月被核酸纯化巨头qiagen(凯杰)收购。enzymatics是生命科学和临床应用领域二代测序和其他遗传分析技术领域方面的酶供应商。公司通过iso13485和9001体系标准认证,并符合fda 21 cfr.820质量系统法规中对常规用途试剂的生产规范产品,其产品占全球二代测序酶品市场75%以上,为全球科研客户提供全面,专业,高效的酶品解决方案。


 

enzymatics酶品包括 超纯连接酶、超纯聚合酶、常规限制性内切酶、修饰酶、rna类酶,核苷酸、pcr及rt-qpcr产品、ngs文库构建试剂盒等,满足广大客户不同研究需要。

现蚂蚁淘生物诚邀您的加入,携手推广enzymatics完美酶品,为广大国内科研用户带来新一代工具酶类产品体验,实现销售佳绩,共创美好未来!

自营商城图标
厂家直采
全球直采模式 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔
在线客服
客服电话

4000-520-616

0512-67156496